by Ellen McGlynn
Though the therapeutic use of wheatgrass has been in practice for many   decades and has gained almost a cult following among proponents of   alternative medicine, it has received its fair share of negative commentary   from conventional health professionals due to its lack of controlled   scientific studies.  Fortunately, in recent years, various research   institutions around the world have ventured to discover the truth about this   mysterious green substance, all with supporting evidence that wheatgrass   really “does a body good.”
What health benefits does fresh wheatgrass juice offer?
In a 2004 study conducted by researchers at an Indian pediatric unit,   positive effects were found in 50% of anemic patients who took 100mL (3.38   ounces) of fresh wheatgrass juice daily for over a year, reducing transfusion   requirements in some patients by as much as 40% (1).   In a   randomized, double-blind, placebo-controlled study at a gastroenterology unit   covering three major Israeli cities, significant reductions in overall   disease activity and rectal bleeding were found in ulcerative colitis   patients who consumed 100mL of fresh wheatgrass juice daily for a month   (2).  In a 1999 Finnish study of rheumatoid arthritis patients who were   subjected to a living foods diet that included wheatgrass juice, patients not   only subjectively reported improvements in their conditions, but their serum   parameters and fecal analyses confirmed it, suggesting that a living foods   diet (of which wheatgrass is often a major part) may lead to a lessening of   not just one but several health risk factors including cardiovascular   diseases and cancer (3). 
What makes fresh wheatgrass so effective?
It is still speculative as to what exactly makes fresh wheatgrass so   effective.  While wheatgrass is full of vitamins and minerals, it is   also very high in chlorophyll.  One ounce of fresh wheatgrass juice   contains 4-12mg of chlorophyll (4). The effects of chlorophyll and its   extracts have been studied extensively since the 1950’s and have proven   beneficial for a wide range of health issues including wound healing and   internal body odor (5), liver cancer caused by food-induced toxins (6,7,8),   colon cancer caused by food-induced toxins (10,15), and the carcinogenic   effects of environmental toxins such as cigarette smoke, coal dust, and   diesel emissions (19).  Though scientists have tried to isolate the   micronutrients and phytochemicals responsible for the effectiveness of   high-chlorophyll foods, it is agreed that whole plant-based foods offer a   healthier and more effective delivery system (10,11,12,14) as well as a more   practical approach to disease prevention (6).
What are the main nutrients in wheatgrass juice?
Wheatgrass juice is actually 95% water.  On average, it also contains   about 2% protein.  The remaining 3% contains over 80 other   nutrients.  According to the Irvine Analytical Laboratory report   courtesy of Optimum Health Institute in California, one ounce of indoor-grown   fresh wheatgrass juice contains approximately 2.86mcg Biotin, 122IU Vitamin   A, .3mcg Vitamin B-12, 4.3IU Vitamin E, 1mg Vitamin C, 8.3mcg Folic Acid,   21.4mg Phosphorus, 8mg Magnesium, .66mg Iron, 7.2mg Calcium, and 42mg   Potassium (4). 
How does fresh wheatgrass juice compare with other   vegetables and natural supplements?
In a 2006 study by the Chemistry Department at the University of Pune in   Pune, India, antioxidant activity was measured in fresh wheatgrass grown   under various conditions and extracted for comparison against a commercially   available wheatgrass tablet.  A higher level of antioxidant activity was   found in the soil-grown (3990 mmol/100g) as opposed to the hydroponically-grown (3740 mmol/100g) wheatgrass   juice, although the variation was not considered significant.  Additionally, the oxygen radical absorbance capacity (ORAC) on   day 10 of growth for both hydroponically-grown  and soil-grown  wheatgrass was higher than ORAC units reported for the wheatgrass   tablet (1380 mmol/100g) as well as many other high-ORAC fruits and vegetables.   (13,16)
| 
ORAC Units per 100 Grams (About 3.5 Oz.) | 
     | 
Wheatgrass Juice | 
3990 | 
 | 
Kale | 
1770 | 
     | 
Blueberries | 
2400 | 
 | 
Strawberries | 
1540 | 
     | 
Blackberries | 
2036 | 
 | 
Spinach | 
1260 | 
     | 
Garlic | 
1940 | 
 | 
Raspberries | 
1220 | 
High-ORAC foods are known to help raise the antioxidant power   of human blood, provide protection from cell damage, and slow processes associated with aging (16,17,18).
Will wheatgrass interfere with my medications?
As chlorophyll has shown to have detoxifying and blood-clotting effects,   wheatgrass juice may interfere with certain  pharmaceuticals, particularly anticoagulants.  Consult your physician about the use of wheatgrass   juice in combination with other drugs.   It may be best to reserve   wheatgrass for use only BEFORE or AFTER specific drug treatments. 
Are there any side effects from fresh wheatgrass juice?
There are no known reports of wheatgrass or chlorophyll toxicity.  Some   people may experience darkened stools or mild stomach upset when first   starting a wheatgrass juice regimen.
How is wheatgrass prepared for consumption?
Humans do not produce the enzyme cellulase required to properly digest the   cellulose structure of grass. Wheatgrass must be juiced.  This requires   the use of a special wheatgrass juicer, which has a gentler extraction   mechanism than regular juicers.  Wheatgrass juicers may also be used for   juicing soft berries, which can be blended with wheatgrass juice to improve   the taste.         
How often should I drink wheatgrass juice?
Taken as a preventative, an ounce a day may be sufficient.    Therapeutically, effects have been experienced by taking three to four ounces   per day.  Juice should be taken on an empty stomach one hour before   meals.  
How much juice can be had from 1 pound of fresh-cut wheatgrass? 
Ten to 12 ounces of juice per pound of cut wheatgrass can   be expected from a good wheatgrass juicer. 
How long will the cut wheatgrass stay fresh?
Fresh-cut wheatgrass can stay fresh in the refrigerator  for up to two   weeks.  
How long does wheatgrass juice stay fresh?
The chemical structure of fresh wheatgrass juice is very unstable.  Once   the grass is juiced, its breaks down rather quickly and loses its nutritional   value.   Fresh wheatgrass should be consumed or frozen almost   immediately after juicing.
LITERATURE CITED
1.  Marawaha RK, Bansal D, Kaur S, Trehan A.  Wheat   grass juice reduces transfusion requirement in patients with thalassemia   major: a pilot study.  Indian Petiatr.  2004;Jul;41(7):716-20.
2..  Ben-Arye E, Goldin E, Wengrower D, Stamper A, Kohn R, Berry E.    Wheat grass juice in the treatment of active distal ulcerative colitis: a   randomized double-blind placebo-controlled trial. Scand J Gastroenterol.   2002;Apr;37(4):444-9.
3.  Hanninen O, Rauma AL, Kaartinen K, Nenonen M.  Vegan diet in   physiological health promotion.  Acta Physiol Hung.1999; 86(3-4):171-80.
4.  Meyerowitz S.  Wheatgrass: Nature’s finest medicine.  6th   ed. Summertown (TN):Book; 1999.
5. Oregon State University Linus Pauling Institute. Corvalis (OR); Updated   2005 Dec 21; cited 2006 Oct 3.  Chlorophyll and Chlorophyllin.    Available from: http://lpi.oregonstate.edu/   infocenter/phytochemicals/chlorophylls/.
6.  Egner PA, Wang JB, Zhu YR, Zhang BC, Wu Y, Zhang QN, Qian GS, Kuang   SY, Gange SJ, Jacobson LP, Helzlsouer KJ, Bailey GS, Groopman JD, Kensler   TW.  Chlorophyllin intervention reduces aflatoxin-DNA adducts in   individuals at high risk for liver cancer.  Proc Natl Acad Sci USA.   2001;Dec4;98(25):14601-6.
7.  Kensler TW, Egner PA, Wang JB, Zhu YR, Zhang BC, Qian GS, Kuang SY,   Gange SJ, Jacobson LP, Munoz A, Groopman JD. Strategies for chemoprevention   of liver cancer.  Eur J Cancer Prev. 2002;Aug;11Suppl 2:S58-64.
8.  Egner PA, Munoz A, Kensler TW.    Chemoprevention with chlorophyllin in individuals exposed to dietary   aflatoxin. Mutat Res. 2003;Feb-Mar;523-524:209-16.
9.  Ouameur AA, Marty R, Tajmir-Riahi HA. Human serum albumin complexes   with chlorophyll and chlorophyllin. Biopolymers. 2005;Feb15;77(3):129-36.
10.  de Vogel J, Jonker-Termont DS, Katan MB, van der Meer R. Natural   chlorophyll but not chlorophyllin prevents heme-induced cytotoxic and   hyperproliferative effects in rat colon. J Nutr. 2005;Aug;135(8):1995-2000.
11.  Oregon State University Linus Pauling Institute. Corvalis (OR);   cited 2006 Oct 3.  Plant-Based Foods.  Available from:   http://lpi.oregonstate.edu/ infocenter/foods.html.
12.  Fahey JW, Stephenson KK, Dinkova-Kostova AT, Egner P, Kensler TW,   Talalay P. Chlorophyll, chlorophyllin and related tetrapyrroles are   significant inducers of mammalian phase 2 cytoprotective genes.   Carcinogenesis. 2005;Jul26(7):1247-55.
13.  Kulkarni SD, Tilak JC, Achaarya R, Rajurkar NS, Devasagayam TP,   Reddy AV.  Evaluation of the antioxidant activity of wheatgrass   (Triticum aestivum L.) as a function of growth under different   conditions.  Phytother Res. 2006;Mar;20(3):218-27. 
14.  Sarkar D, Sharma A, Talukder G.  Chlorophyll and chromosome   breakage.  Mutat Res. 1996;Aug8;360(3):187-91.
15.  Diaz GD, Li Q, Dashwood RH.  Capase-8 and apoptosis-inducing   factor mediate a cytochrome c-independent pathway of apoptosis in human colon   cancer cells induced by a dietary phytochemical chlorophyllin.   2003;Mar15;63(6)1254-61.
16.  USDA Agricultural Research Service. Updated 13 Aug 2003.    Cited 6 Oct 2006.  Food & Nutrition Briefs.  Available from:   http://www.ars.usda.gov/is/np/fnrb/fnrb499.htm.
17.  Cao G, Booth SL, Sadowski JA, Prior RL.  Increases in human   plasma antioxidant capacity after consumption of controlled diets high in   fruit and vegetables. Am J Clin Nutr. 1998;68:1081-7.
18.  Cao G, Russel RM, Lischner N, Prior RL. Serum antioxidant capacity   is increased by consumption of strawberries, spinach, red wine or vitamin C   in elderly women.  J Nutr. 1998 Dec;128(12):2383-90.
19.  Ong T, Whong WZ, Stewart J, Brockman HE. Chlorophyllin: a potent   antimutagen against environmental and dietary complex mixtures.  Mutat   Res. 1986 Feb;173(2):111-5.